Porcine C-peptide ELISA(10-1256-01)

Kit format: 1 x 96 wells

Measurement range: 20-1200 pmol/L

• No cross reactivity to human proinsulin or insulin
• No cross reactivity to C-peptide in human, macaque, rat or mouse
• High specificity - enables specific measurement of porcine C-peptide in xenotransplantation studies

An assay for the quantitative determination of porcine C-peptide in serum, plasma and cell culture medium.

The assay may be used for post-transplant assessment of porcine islet xenograft function in human, macaque, rat or mouse. Enables distinguishing endogenously produced C-peptide for pharmacodynamic and/or pharmacokinetic studies during exogenous insulin administration

Test characteristics

Samples: Serum, plasma and cell culture medium
Sample volume: 10 µL
Assay range: 20-1200 pmol/L
Detection limit: 10 pmol/L
Incubation: 2 h + 1 h + 15 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Porcine insulin < 0.05 %
Porcine proinsulin < 0.02 %
Human C-peptide < 0.003 %
Human insulin < 0.002 %
Human proinsulin < 0.01 %
Macaque C-peptide < 0.006 %
Mouse insulin < 0.13 %
Mouse proinsulin < 0.002 %
Rat C-peptide 0.08 %
Rat insulin < 0.002 %
Rat proinsulin < 0.002 %