Ultrasensitive C-peptide ELISA(10-1141-01)

Kit format: 1 x 96 wells

Measurement range: 5 - 280 pmol/L (0.015 – 0.85 ng/mL)

• High sensitivity, detetction limit < 2.5 pmol/L (0.0076 µg/L)
• No or minimal cross-reactivity to insulin or proinsulin
• Calibrated against International Reference Reagent IRR84/510 for human C-peptide.
• CE/IVD labeled on select markets

The Mercodia Ultrasensitive C-peptide ELISA is a high quality immunoassay for specific quantification of human C-peptide in serum, plasma, cell culture media or urine.

Even higher sensitivity can be achieved by using an alternative protocol described in TechNote 0144.

Test characteristics

Samples: Serum, EDTA and heparin plasma as well as urine.
Sample volume: 50 µL
Assay range: 5 - 280 pmol/L (0.015 – 0.85 ng/mL)
Detection limit: 2.5 pmol/L (0.0076 µg/L)
Incubation: 1 h + 1 h + 30 min
Detection method: absorbance

Specificity

The following cross-reactions have been tested:

Insulin < 0.0006 %
Proinsulin 5 %
Proinsulin (Des 31-32) 3 %
Proinsulin (Split 32-33) 2 %
Proinsulin (Des 64-65) 74 %
Proinsulin (Split 65-66) 10 %